在线翻译

Transposon insertional mutagenesis is a basic tool for addressing gene function through analysis of mutant phenotypes and identification of mutated genes in eukaryotic genomes [73–83]. Together with the classical ‘transposon tagging’ approach, other strategies based on insertional mutagenesis have been devised for the identification of genes that do not produce easily observable phenotypes when knocked-out. In the ‘activation tagging’ method [84], strong activating sequences 更多：https://www.bmcx.com/ , such as the cauliflower mosaic virus (CaMV) 35S enhancer, are inserted into transposon sequences. Tagged genes can consequently be overex-pressed resulting in gain-of-function phenotypes. In ‘gene trapping’ and ‘enhancer trapping’, transposon constructs containing a reporter gene that can respond to cis-acting transcriptional signals at the site of insertion are employed. The host gene is identified by observing the reporter gene expression pattern and sequencing of tagged sites. Different trapping systems have been reviewed by Springer [85].