在线翻译

The ligation mediated P CR (L M- PCR) [53] is based on the primer extension of a specific oligonucleotide carried out on a c he mically nicked DNA. A double-strand DNA cassette is t he n ligated to the obtained blunt-end DN A providing the substrate for the final PCR. The linear amplification mediated PCR (LAM-PCR) [54] differs i n the strategy used to obtain the double-strand DNA fragment to ligate with the DNA cassette. In t his case a 5¢ -biotinylated specific primer is ex tended on t he genomic D NA. After capture by strep tavidin beads o f t he ex tension product, a second strand is synthesized by random hexanucleo tide p rim-in g. The double-strand DNA obtained is then digested with a four nucleotide recognizing restriction enzyme and ligated to a p roper DNA cassette. The ligation product i s subjected to a nested PCR amplification, whose product sare further selected by gel electrophoresis analysis 更多：https://www.bmcx.com/ , eluted, re- amplified and sequenced.