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Total Count Purpose This method is intended to determine the number of microorganisms in samples. It is also called the aerobic colony count, standard plate count or total plate count. This methodology can be applied to count different groups of microorganisms by changing the temperatures of growth. Method, Media Method Media* 刪 For filterable products: Membrane Filtration, SM-PR- 625 刪 For non-filterable products: Pour Plate, SM-PR-655 刪 M-TGE (yellow ampoules) 刪 M-TGE (yellow ampoules) with TTC indicator* 刪 Nutrient broth/agar 刪 Plate count broth/agar 刪 Plate count broth with TTC indicator* 刪 Standard count broth/agar 刪 Standard count broth with TTC indicator* *Media contains TTC that causes colonies to turn red and plates are easier to read. *Refer to Media Preparation and Handling, SM-PR-641, for preparing culture media. Microorganism, Incubation Microorganism Incubation Mesophilic Total Count 刪 Incubate at 35 ∮ 2 C for 72 hours. 刪 For liquid milk ingredients, incubate at 32 ∮ 1 C for 48 hours. For dry milk ingredients 更多：https://www.bmcx.com/ , incubate plates at 32 ∮ 1 C for 72 hours (or follow local regulations). Total Count Standard Methods: Microbiological SM-PR-685 Revision: 03-Dec-2010 Page 2 of 2 Classified - Internal use Microorganism Incubation Psychrotrophic Total Count 刪 Incubate at 7 ∮ 1 C for 10 days. 刪 Alternatively, incubation for 16 hours at 17 ∮ 1o C followed by incubation for 3 days at 7 ∮ 1o C. Thermophilic Total Count 刪 Incubate at 55  ∮ 2 C for 48-72 hours. 刪 Maintain humidity during incubation to prevent drying of agar medium. Interpretation 刪 On all media types, bacteria develop a wide range of colored colonies, including transparent. Texture of the colonies range from ¨slimy〃 to a ¨powdery〃 appearance. Colony shape may vary from flat spreading to mounding clumping forms. Mold and some yeast may also grow on these plates presenting different colors, texture and shape. 刪 Count all colonies present on the Petri dish, (including bacteria, mold and yeasts). Report as number of Psychrotrophic, Mesophilic or Thermophilic CFU per sample size. Revision History Revision Date Summary of Change 03-Dec-2010 Reviewed and revised for migration to the KORE Library. 31-Mar-2006 New document, incorporated information from SM-PR-660.