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The ligation mediated PCR (LM- PCR) [53] is based on the primer extension of a specific oligonucleotide carried out on a chemically nicked DNA. A double-strand DNA cassette is then ligated to the obtained blunt-end DNA providing the substrate for the final PCR. The linear amplification mediated PCR (LAM-PCR) [54] differs in the strategy used to obtain the double-strand DNA fragment to ligate with the DNA cassette. In this case a 5¢ -biotinylated specific primer is extended on the genomic DNA. After capture by strep tavidin beads of the extension product, a second strand is synthesized by random hexanucleo tide prim-ing. The double-strand DNA obtained is then digested with a four nucleotide recognizing restriction enzyme and ligated to a proper DNA cassette. The ligation product is subjected to a nested PCR amplification, whose product sare further selected by gel electrophoresis analysis 更多：https://www.bmcx.com/ , eluted, re- amplified and sequenced.